Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34929
Title: Intein-mediated attachment of ligands to proteins for immobilization onto a support
Authors: LESAICHERRE, MARIE-LAURE 
LUE, YEE PENG RINA 
UTTAMCHANDANI, MAHESH 
CHEN, Y.J. GRACE 
YAO, SHAO QIN 
Issue Date: 30-Dec-2004
Source: LESAICHERRE, MARIE-LAURE,LUE, YEE PENG RINA,UTTAMCHANDANI, MAHESH,CHEN, Y.J. GRACE,YAO, SHAO QIN (2004-12-30). Intein-mediated attachment of ligands to proteins for immobilization onto a support. ScholarBank@NUS Repository.
Abstract: The present invention provides an intein-mediated method of attaching a ligand to a protein for immobilization onto a support functionalized with an affinity receptor. In one embodiment, the ligand is biotin and the affinity receptor is avidin. Biotin is attached to the protein by reacting cysteine-biotin with a fusion protein comprising a cleavable intein and a protein of interest to effect cleavage of the cystein and attachment of biotin to the protein. The present invention further provides a novel protein array and a high throughput method of preparing protein arrays by expressing the protein of interest as an intein fusion protein including a binding domain for purification, attaching a ligand to the fusion protein under condition suitable for cleavage of the intein and attachment of the ligand to the remaining protein and immobilizing the resulting protein-ligand onto a support that has been functionalized with an affinity receptor.
URI: http://scholarbank.nus.edu.sg/handle/10635/34929
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