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https://scholarbank.nus.edu.sg/handle/10635/34877
Title: | SUSHI PEPTIDE MULTIMER | Authors: | DING, JEAK LING HO, BOW |
Issue Date: | 12-Apr-2006 | Citation: | DING, JEAK LING,HO, BOW (2006-04-12). SUSHI PEPTIDE MULTIMER. ScholarBank@NUS Repository. | Abstract: | Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimick other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coli. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS-induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS. | URI: | http://scholarbank.nus.edu.sg/handle/10635/34877 |
Appears in Collections: | Staff Publications |
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