Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34379
Title: Antigen-specific Effector CD8 T Cells Regulate Allergic Responses via IFN-y and Dendritic Cell Function
Authors: TANG YAFANG
Keywords: antigen-specific, CD8 T cells, dendritic cells, IFN-gamma, asthma, mouse
Issue Date: 19-Jan-2012
Source: TANG YAFANG (2012-01-19). Antigen-specific Effector CD8 T Cells Regulate Allergic Responses via IFN-y and Dendritic Cell Function. ScholarBank@NUS Repository.
Abstract: In this study, we aim to investigate the potential of CD8 T cells to modulate the elicitation of allergic inflammation and investigate the underlying mechanisms. Effector CD8 T cells from OT-I and IFN-gamma-/-OT-I mice were transferred to OVA-sensitized mice one day before OVA challenges. The effect on lung dendritic cells (DCs) exerted by CD8 T cells was studied with ex vivo culture of sorted DCs from treatment mice with either naive or antigen-experienced CD4 T cells. We found that effector OT-I, but not IFN-gamma-/-OT-I CD8 T cells attenuated eosinophilia and mucus secretion in the lungs of sensitized mice in an antigen-specific manner. Effector IFN-gamma-/-OT-I CD8 T cells displayed a Tc2/Tc17-biased phenotype with weaker cytotoxicity and were able to both induce and exacerbate eosinophilia as well as neutrophilia. OT-I CD8 T cells increased the ability of lung CD11b+CD103- DCs to both prime the differentiation of naive CD4 T cells toward a Th1 phenotype and enhance IFN-gamma production by antigen-experienced lung CD4 T cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/34379
Appears in Collections:Ph.D Theses (Open)

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