Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34346
Title: Synthesis of Near-infrared Cyanine Dye Library with Increased Photostability and its Application in Fluorescence and SERS Imaging
Authors: ANIMESH SAMANTA
Keywords: Cyanine, photostable, bio-imaging, SERS, multiplexing, sensitive
Issue Date: 17-Jan-2012
Source: ANIMESH SAMANTA (2012-01-17). Synthesis of Near-infrared Cyanine Dye Library with Increased Photostability and its Application in Fluorescence and SERS Imaging. ScholarBank@NUS Repository.
Abstract: With the emerging interest in optical in vivo imaging, there is an increasing demand of photostable near-infrared (NIR) dyes. Therefore, we applied a combinatorial approach to identify CyNA-414 as the most photostable among 80 photostable tricarbocyanine dyes (CyNA). Next, we modified CyNA-414 for bioconjugation (CyNE790) and proved its superiority considering its photophysical and photostability properties compared with standard ICG. The superior in vivo imaging was further confirmed by injecting CyNE790-anti-EGFR into mice. Moreover, we developed a novel NIR fluorescent deoxyglucose analogue (CyNE-2DG) and proved its superior cell permeability and cellular imaging over the NIR standard IRDye800CW-2DG. However, we studied another good application of cyanine dyes in Surface-enhanced Raman Scattering (SERS). We employed a combinatorial approach to identify CyNAMLA-381 as highly sensitive NIR SERS reporters among 80 dyes. Then, we demonstrated ultrasensitive tumor detection in vivo by applying the nanotags (AuNPs-CyNAMLA-381-anti-HER2). Moreover, we designed a multiplexing partner of Raman reporters to enable the multiplex recognition of two different cancer cells with antibody-conjugated nanotags. We also demonstrated the multiplexing capability of three NIR Raman reporters in vivo.
URI: http://scholarbank.nus.edu.sg/handle/10635/34346
Appears in Collections:Ph.D Theses (Open)

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