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|Title:||Molecular cloning of a cardiotoxin structural gene from Malayan spitting cobra (Naja naja sputatrix)|
|Citation:||Yeo, M.S.L., Jeyaseelan, K., Chung, M.C.M., Gopalakrishnakone, P., Tan, C.H., Wong, H.A. (1993). Molecular cloning of a cardiotoxin structural gene from Malayan spitting cobra (Naja naja sputatrix). Toxicon 31 (1) : 53-60. ScholarBank@NUS Repository. https://doi.org/10.1016/0041-0101(93)90356-N|
|Abstract:||The structural gene and cDNA encoding cardiotoxin in Naja naja sputatrix have been cloned and characterized with a view to study the gene protein relationships and also to produce pure protein in large amounts. Using the polymerase chain reaction on the total RNA isolated from the venom glands, the structural gene (180 bp) has been synthesized and expressed in Escherichia coli to produce a fusion protein with β-galactosidase. Immunoblotting using polyclonal antibodies raised against the total venom in rabbits demonstrated the presence of cross-reacting proteins in plaques produced by recombinant λgt11 phages.|
|Appears in Collections:||Staff Publications|
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