Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/32654
Title: Assays for endotoxin
Authors: DING, JEAK LING 
HO, BOW 
Issue Date: 11-Nov-2003
Source: DING, JEAK LING,HO, BOW (2003-11-11). Assays for endotoxin. ScholarBank@NUS Repository.
Abstract: The horseshoe crab, Carcinoscorpius rotundicauda Factor C cDNA (CrFC21) has been cloned into a shuttle baculoviral vector and another vector suitable for expression in insect cells. The recombinant baculoviral DNA was then transfected into the insect cells for expression of recombinant Factor C. Recombinant Factor C was found to be immunoreactive and is capable of binding both free and bound/immobilized lipid A. It is enzymatically active when triggered by LPS. The rFC is probably of the two-chain form, being cleaved into the heavy and light chains after activation by Gram negative bacterial endotoxin. As low as 0.01 pg (0.001 ng/ml) of LPS was detectable by the rFC, thus, indicating its potentials as a novel generation of "limulus amoebocyte lysate."
URI: http://scholarbank.nus.edu.sg/handle/10635/32654
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