A UNIQUE CLOSTRIDIUM SPECIES THAT PRODUCES BIOFUELS FROM MONOSACCHARIDES AND POLYSACCHARIDES.

Abstract

A unique mesophilic Clostridium species strain BOH3 was obtained in this study, which is capable of fermenting monosaccharides to produce butanol and hydrolyzing polysaccharides to produce hydrogen (H2) and volatile fatty acids (VFAs). From 30 g/L of glucose and xylose each, batch culture BOH3 was able to produce 4.67 and 4.63 g/L of butanol. Enhancement treatments by increasing the inoculated cells improved butanol production to 7.05 and 7.41 g/L, respectively. This culture also showed its ability to withstand high concentration of butanol, when exogenous butanol was added. Hydrogen production (2.47 and 1.93 mmol) was observed when cellulose and xylan (10 g/L each) were used, suggesting that strain BOH3 possesses xylanolytic and cellulolytic capabilities. In term of practical application, this culture showed its ability to directly convert food waste to butanol, with the absence of separate delignification and enzymatic hydrolysis. These unique features reveal the strain¿s novelty as most wild-type solventogenic strains have not been reported to have such properties. Therefore, culture BOH3 is promising in generating butanol and hydrogen from renewable feedstock.