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|Title:||Effects of recombinant human interferon alpha on aryl hydrocarbon hydroxylase activity in cultured human peripheral lymphocytes|
|Authors:||Moochhala, S.M. |
|Citation:||Moochhala, S.M., Lee, E.J.D. (1991). Effects of recombinant human interferon alpha on aryl hydrocarbon hydroxylase activity in cultured human peripheral lymphocytes. Life Sciences 48 (18) : 1715-1719. ScholarBank@NUS Repository. https://doi.org/10.1016/0024-3205(91)90207-R|
|Abstract:||Interferon and its inducers are known to depress drug biotransformation in vivo by decreasing the levels of cytochrome P-450 (P450) monooxygenase systems in the liver. However, very little is known about the effects of interferon on P450 in extrahepatic tissues. In this study we investigated the effects of a recombinant human interferon-alpha (rhIFN-α) on aryl hydrocarbon hydroxylase (P450IAI) in cultured human peripheral lymphocytes (HPL). Non-induced and induced (3-methylcholantherene) mitogen activated lymphocytes were used throughout the study. rhIFN-α maximally depressed AHH activity to approximately 58% of control after 24 hrs of incubation in both non-induced and induced lymphocytes. However, after 48 hrs of incubation with rhIFN-α, AHH activity had recovered to 86% of control in induced cells and 61% in non-induced cells. rhIFN-α had no significant effect on either NADH cytochrome c reductase activity or on viable lymphocyte cell count. This is the first demonstration that rhIFN-α can have a direct depressive effect on a P450 dependent monooxygenase system in HPL.|
|Source Title:||Life Sciences|
|Appears in Collections:||Staff Publications|
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