Please use this identifier to cite or link to this item: https://doi.org/10.1016/0024-3205(91)90207-R
Title: Effects of recombinant human interferon alpha on aryl hydrocarbon hydroxylase activity in cultured human peripheral lymphocytes
Authors: Moochhala, S.M. 
Lee, E.J.D.
Issue Date: 1991
Source: Moochhala, S.M.,Lee, E.J.D. (1991). Effects of recombinant human interferon alpha on aryl hydrocarbon hydroxylase activity in cultured human peripheral lymphocytes. Life Sciences 48 (18) : 1715-1719. ScholarBank@NUS Repository. https://doi.org/10.1016/0024-3205(91)90207-R
Abstract: Interferon and its inducers are known to depress drug biotransformation in vivo by decreasing the levels of cytochrome P-450 (P450) monooxygenase systems in the liver. However, very little is known about the effects of interferon on P450 in extrahepatic tissues. In this study we investigated the effects of a recombinant human interferon-alpha (rhIFN-α) on aryl hydrocarbon hydroxylase (P450IAI) in cultured human peripheral lymphocytes (HPL). Non-induced and induced (3-methylcholantherene) mitogen activated lymphocytes were used throughout the study. rhIFN-α maximally depressed AHH activity to approximately 58% of control after 24 hrs of incubation in both non-induced and induced lymphocytes. However, after 48 hrs of incubation with rhIFN-α, AHH activity had recovered to 86% of control in induced cells and 61% in non-induced cells. rhIFN-α had no significant effect on either NADH cytochrome c reductase activity or on viable lymphocyte cell count. This is the first demonstration that rhIFN-α can have a direct depressive effect on a P450 dependent monooxygenase system in HPL.
Source Title: Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/32123
ISSN: 00243205
DOI: 10.1016/0024-3205(91)90207-R
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