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Title: Location of renin-angiotensin system components in the hypoglossal nucleus of the rat
Authors: Tham, M.
Sim, M.K. 
Tang, F.R.
Keywords: ACE
Angiotensin AT1receptor
Electron microscopy
Light microscopy
Issue Date: 2001
Citation: Tham, M., Sim, M.K., Tang, F.R. (2001). Location of renin-angiotensin system components in the hypoglossal nucleus of the rat. Regulatory Peptides 101 (1-3) : 51-57. ScholarBank@NUS Repository.
Abstract: The renin-angiotensin system (RAS) in the hypoglossal nuclei of the rat was studied by immunohistochemistry. Antibodies to angiotensin AT1 receptor (AT1), angiotensinogen (ANG), renin (REN), angiotensin converting enzyme (ACE) and angiotensin II (AII) were used. All the components of the RAS with the exception of renin were detected. Light and electron microscopy revealed the following results: ANG was predominantly found in astrocytes, with small amounts in neuronal dendrites; ACE was found in the cytoplasm of neurons, dendrites and astrocyte processes; AT1 was found in the cytoplasm of neurons and dendrites, but not on the membrane; and AII was found mainly in astrocytes with some located in the dendrites and cytoplasm. Right hypoglossal nerve lesion caused an increase in expression of AT1 in neurons as early as 2 days post-lesion. An increase in expression of ANG in astrocytes was also seen, but at a much later time of 3 weeks post-lesion. For AII, staining occurred in both the neurons and astrocytes in the undamaged hypoglossal nucleus. Nerve lesion caused a disappearance of neuronal stains and an increase in astrocyte stains. There were no changes in ACE staining after nerve lesion. We speculate that ANG and AII are made within the astrocytes, whereas ACE could either be uptaken from blood or de novo synthesized. AT1 may potentially be internal soluble receptors. As to the function of AII in the hypoglossal nucleus, the data do not support AII as a neurotransmitter in the hypoglossal nucleus. It may function as a neuromodulator and also be involved in basic cellular activities, e.g. regulation of transcription factors. © 2001 Elsevier Science B.V. All rights reserved.
Source Title: Regulatory Peptides
ISSN: 01670115
DOI: 10.1016/S0167-0115(01)00260-9
Appears in Collections:Staff Publications

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