Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-4347(94)00458-H
Title: Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion
Authors: Wu, J.
Zhou, C.Y.
Chi, H.
Wong, M.K. 
Lee, H.K. 
Ong, H.Y.
Ong, C.N. 
Issue Date: 1995
Source: Wu, J., Zhou, C.Y., Chi, H., Wong, M.K., Lee, H.K., Ong, H.Y., Ong, C.N. (1995). Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion. Journal of Chromatography B: Biomedical Applications 663 (2) : 247-253. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-4347(94)00458-H
Abstract: An ion-pair reversed-phase high-performance liquid chromatographic method with fluorimetric detection, using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid] as a ligand, has been successfully applied to the determination of aluminium in human serum. The highly fluorescent aluminium-lumogallion complex (λ(ex) 505 nm, λ(em) 574 nm) was separated on a LiChrosorb RP-18 column with an eluent consisting of 30% acetonitrile, 70% 0.02 M potassium hydrogen phthalate and 10 μM lumogallion. The proposed system offers a simple, rapid, selective and sensitive method for the determination of aluminium in serum. The detection limit for aluminium was 0.05 μg/l in aqueous solution and the limit of determination was 2.2 μg/l in serum. The recovery of the method is generally over 90%.
Source Title: Journal of Chromatography B: Biomedical Applications
URI: http://scholarbank.nus.edu.sg/handle/10635/32048
ISSN: 15726495
DOI: 10.1016/0378-4347(94)00458-H
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