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|Title:||Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion|
|Citation:||Wu, J., Zhou, C.Y., Chi, H., Wong, M.K., Lee, H.K., Ong, H.Y., Ong, C.N. (1995). Determination of serum aluminium using an ion-pair reversed-phase high-performance liquid chromatographic-fluorimetric system with lumogallion. Journal of Chromatography B: Biomedical Applications 663 (2) : 247-253. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-4347(94)00458-H|
|Abstract:||An ion-pair reversed-phase high-performance liquid chromatographic method with fluorimetric detection, using lumogallion [4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulphonic acid] as a ligand, has been successfully applied to the determination of aluminium in human serum. The highly fluorescent aluminium-lumogallion complex (λ(ex) 505 nm, λ(em) 574 nm) was separated on a LiChrosorb RP-18 column with an eluent consisting of 30% acetonitrile, 70% 0.02 M potassium hydrogen phthalate and 10 μM lumogallion. The proposed system offers a simple, rapid, selective and sensitive method for the determination of aluminium in serum. The detection limit for aluminium was 0.05 μg/l in aqueous solution and the limit of determination was 2.2 μg/l in serum. The recovery of the method is generally over 90%.|
|Source Title:||Journal of Chromatography B: Biomedical Applications|
|Appears in Collections:||Staff Publications|
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