Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-4347(91)80311-Y
Title: Determination of thiamine and its phosphate esters by gradient-elution high-performance liquid chromatography
Authors: Lee, B.L.
Ong, H.Y.
Ong, C.N. 
Issue Date: 1991
Source: Lee, B.L., Ong, H.Y., Ong, C.N. (1991). Determination of thiamine and its phosphate esters by gradient-elution high-performance liquid chromatography. Journal of Chromatography - Biomedical Applications 567 (1) : 71-80. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-4347(91)80311-Y
Abstract: A high-performance liquid chromatographic method for the determination of thiamine and its phosphate esters using gradient elution is presented. Chromatography was performed on an octadecyl C18 column with post-column derivatization using potassium ferricyanide: thiamine was oxidized to thiochrome, which was detected fluorimetrically. The gradient system avoids the use of a modifier and an ion-pairing reagent. The proposed method enables the separation of thiamine triphosphate, thiamine pyrophosphate, thiamine monophosphate and free thiamine, at concentrations of ca. 2 nM, within 10 min. The within-assay precision for blood and serum total thiamine was less than 2 and 5%, respectively, and the between-day variation was less than 3 and 5%, respectively. The method is simple and rapid, and it can be used for screening of thiamine deficiency and for clinical study of various diseases related to vitamin B1 deficiency.
Source Title: Journal of Chromatography - Biomedical Applications
URI: http://scholarbank.nus.edu.sg/handle/10635/31868
ISSN: 03784347
DOI: 10.1016/0378-4347(91)80311-Y
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

18
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

15
checked on Dec 13, 2017

Page view(s)

164
checked on Dec 9, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.