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Title: Determination of thiamine and its phosphate esters by gradient-elution high-performance liquid chromatography
Authors: Lee, B.L.
Ong, H.Y.
Ong, C.N. 
Issue Date: 1991
Citation: Lee, B.L., Ong, H.Y., Ong, C.N. (1991). Determination of thiamine and its phosphate esters by gradient-elution high-performance liquid chromatography. Journal of Chromatography - Biomedical Applications 567 (1) : 71-80. ScholarBank@NUS Repository.
Abstract: A high-performance liquid chromatographic method for the determination of thiamine and its phosphate esters using gradient elution is presented. Chromatography was performed on an octadecyl C18 column with post-column derivatization using potassium ferricyanide: thiamine was oxidized to thiochrome, which was detected fluorimetrically. The gradient system avoids the use of a modifier and an ion-pairing reagent. The proposed method enables the separation of thiamine triphosphate, thiamine pyrophosphate, thiamine monophosphate and free thiamine, at concentrations of ca. 2 nM, within 10 min. The within-assay precision for blood and serum total thiamine was less than 2 and 5%, respectively, and the between-day variation was less than 3 and 5%, respectively. The method is simple and rapid, and it can be used for screening of thiamine deficiency and for clinical study of various diseases related to vitamin B1 deficiency.
Source Title: Journal of Chromatography - Biomedical Applications
ISSN: 03784347
DOI: 10.1016/0378-4347(91)80311-Y
Appears in Collections:Staff Publications

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