Please use this identifier to cite or link to this item:
|Title:||Involvement of reactive oxygen species in aflatoxin B1-induced cell injury in cultured rat hepatocytes|
|Authors:||Shen, H.-M. |
Reactive oxygen species
|Citation:||Shen, H.-M., Ong, C.-N., Shi, C.-Y. (1995). Involvement of reactive oxygen species in aflatoxin B1-induced cell injury in cultured rat hepatocytes. Toxicology 99 (1-2) : 115-123. ScholarBank@NUS Repository. https://doi.org/10.1016/0300-483X(94)03008-P|
|Abstract:||The role of reactive oxygen species (ROS) in AFB1-induced cell injury was investigated using cultured rat hepatocytes. Malonaldehyde (MDA) generation and Tactate dehydrogenase (LDH) release were determined as indices of lipid peroxidation and cell injury, respectively. Exposure to AFB1 for up to 72 h resulted in significantly elevated levels of LDH being released into the medium as well as the MDA generation in cultured hepatocytes. These effects were dose-dependent, indicating that AFB1 was capable of inducing oxidative damages in the cell. Further, MDA generation and LDH release were effectively inhibited by the addition of the following: (1) superoxide dismutase (500 units/ml), (2) catalase (1500 units/ml), (3) 10 mM desferrioxamine (a specific iron chelator), or (4) 260 mM dimethyl sulfoxide (a hydroxyl radical scavenger). These evidences therefore suggest that ROS, such as superoxide radicals, hydroxyl radicals and hydrogen peroxides, are involved in AFB1-induced cell injury in cultured rat hepatocytes.|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 21, 2019
WEB OF SCIENCETM
checked on Feb 12, 2019
checked on Jan 13, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.