Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0166-0934(01)00431-1
Title: Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma
Authors: Xiang, X. 
Qiu, D. 
Chan, S.H. 
Tan, W.C. 
Chan, K.P.
Hegele, R.G.
Keywords: Nasopharyngeal aspirates
Respiratory virus
RT-PCR
Sputum
Issue Date: 2002
Source: Xiang, X., Qiu, D., Chan, S.H., Tan, W.C., Chan, K.P., Hegele, R.G. (2002). Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma. Journal of Virological Methods 101 (1-2) : 127-133. ScholarBank@NUS Repository. https://doi.org/10.1016/S0166-0934(01)00431-1
Abstract: Viral respiratory tract infections are associated frequently with acute exacerbations of asthma. Nasopharyngeal aspirates and bronchoalveolar lavage specimens are used extensively for detecting viral respiratory tract infections, but not sputum. The aim of the study was to determine the efficiency of viral detection in induced sputum versus nasopharyngeal aspirate obtained during acute exacerbations of asthma, comparing three laboratory methods of viral diagnosis. Paired samples of induced sputum and nasopharyngeal aspirate obtained from 32 adults admitted to hospital with acute asthma were subjected to reverse transcription-polymerase chain reaction (RT-PCR), viral culture, and immunofluorescence assay. The results show that RT-PCR was associated with significantly higher rates of viral detection than culture (P = 0.005) or immunofluorescence (P = 0.001), without significant differences in the rates of viral detection between induced sputum and nasopharyngeal aspirate. It is concluded that induced sputum specimens are feasible for detection of viral respiratory tract infections by RT-PCR during acute exacerbations of asthma. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Journal of Virological Methods
URI: http://scholarbank.nus.edu.sg/handle/10635/31512
ISSN: 01660934
DOI: 10.1016/S0166-0934(01)00431-1
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