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https://scholarbank.nus.edu.sg/handle/10635/31494
| Title: | Serum IgG response to differentiated antigens of Helicobacter pylori | Authors: | Hua, J.-S. Khin, M.M. Zheng, P.-Y. Yeoh, K.-G. Ng, H.-C. Ho, B. |
Keywords: | Antigen-antibody reactions Antigens, bacterial Antigens, differentiation Coccoid form ELISA Gastritis Helicobacter pylori Helicobacter pylori infection IgG Spiral form Stomach neoplasms Stomach ulcer |
Issue Date: | 1998 | Citation: | Hua, J.-S., Khin, M.M., Zheng, P.-Y., Yeoh, K.-G., Ng, H.-C., Ho, B. (1998). Serum IgG response to differentiated antigens of Helicobacter pylori. World Journal of Gastroenterology 4 (1-6) : 249-251. ScholarBank@NUS Repository. | Abstract: | AIM: To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS: Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H. pylori on chocolate blood agar was used to provide spiral form. 'Synchronous' coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0.4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme-linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori. RESULTS: Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non-ulcer dyspepsia (NUD) (44%) and other diseases (45%) (P | Source Title: | World Journal of Gastroenterology | URI: | http://scholarbank.nus.edu.sg/handle/10635/31494 | ISSN: | 10079327 |
| Appears in Collections: | Staff Publications |
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