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https://doi.org/10.1016/j.vaccine.2003.10.017
| Title: | Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae | Authors: | Seah, J.N. Kwang, J. |
Keywords: | Actinobacillus pleuropneumoniae Neutrophil RTX toxin |
Issue Date: | 2004 | Citation: | Seah, J.N., Kwang, J. (2004). Localization of linear cytotoxic and pro-apoptotic epitopes in RTX toxin ApxIII of Actinobacillus pleuropneumoniae. Vaccine 22 (11-12) : 1494-1497. ScholarBank@NUS Repository. https://doi.org/10.1016/j.vaccine.2003.10.017 | Abstract: | RTX toxin ApxIII secreted by Actinobacillus pleuropneumoniae affects porcine pulmonary alveolar macrophages and neutrophils. The distribution of ApxIII linear cytotixic and pro-apoptotic determinants was characterized by neutrophil protection assay, DNA fragmentation and caspase-3 activity using antisera produced against its N-terminus, hydrophobic domain, activation domain, calcium-binding domain and C-terminal half. Neutralization of ApxIII cytotoxicity and pro-apoptotic acitivities was found in antisera raised against its N- and C-termini whereas ApxIII activation domain-specific antiserum expressed cytotoxic-neutralizing activity. No neutralizing activities were detected in antisera against other structural domains of ApxIII suggesting that the putative linear cytotoxic and pro-apoptotic determinants of ApxIII were mapped to its terminal domains and activation domains. © 2003 Elsevier Ltd. All rights reserved. | Source Title: | Vaccine | URI: | http://scholarbank.nus.edu.sg/handle/10635/31288 | ISSN: | 0264410X | DOI: | 10.1016/j.vaccine.2003.10.017 |
| Appears in Collections: | Staff Publications |
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