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Title: Effects of Hydrogen Peroxide on Different Models of Wound Healing
Keywords: Wound healing, hydrogen peroxide, H2O2, ROS, oxidative damage, MAPK
Issue Date: 17-Aug-2011
Citation: LOO ENG KIAT ALVIN (2011-08-17). Effects of Hydrogen Peroxide on Different Models of Wound Healing. ScholarBank@NUS Repository.
Abstract: It has been established that low concentrations of H<sub>2</sub>O<sub>2</sub> are produced in wounds. Yet at the same time, there is evidence that excessive oxidative damage is correlated with chronic wounds. In this thesis we explored the effects of H<sub>2</sub>O<sub>2</sub> in keratinocyte cell culture models and an <i>in vivo</i> excision wound model of wound healing. H<sub>2</sub>O<sub>2</sub> stimulates a persistent ERK phosphorylation in HaCaT keratinocytes which was found to be important in cell proliferation and migration. H<sub>2</sub>O<sub>2</sub> also increases the production of proinflammatory and pro-angiogenic cytokines such as Vascular endothelial growth factor, Interleukin-8, Granulocyte-macrophage colony-stimulating factor, Tumor necrosis factor-a, interleukin-6 and Interferon gamma-induced protein 10, in HaCaT keratinocytes. H<sub>2</sub>O<sub>2</sub> was found to increase re-epithelialization in a primary fibroblast-keratinocyte co-culture model as well. In a C57BL/6 mice excision wound model, low concentrations of H<sub>2</sub>O<sub>2</sub> (10 mM) were found to enhance angiogenesis while high concentrations of H<sub>2</sub>O<sub>2</sub> (166 mM) retarded wound closure and connective tissue formation. High concentrations of H<sub>2</sub>O<sub>2</sub> also increased the levels of MMP-8 in the wounds, which could be the cause of reduced connective tissue formation. Wounding was found to increase oxidative lipid damage, as measured by F<sub>2</sub>-isoprostanes, but H<sub>2</sub>O<sub>2</sub> treatment does not significantly increase it even at concentrations that delay wound healing. This challenges the putative claim that oxidative damage contributes to the pathology of poor healing wounds.
Appears in Collections:Ph.D Theses (Open)

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