Please use this identifier to cite or link to this item:
|Title:||Effects of bacillus Calmette-Guerin and interferon α-2b on cytokine production in human bladder cancer cell lines|
|Authors:||Zhang, Y.E. |
|Source:||Zhang, Y.E., Khoo, H.E., Esuvaranathan, K. (1999). Effects of bacillus Calmette-Guerin and interferon α-2b on cytokine production in human bladder cancer cell lines. Journal of Urology 161 (3) : 977-983. ScholarBank@NUS Repository. https://doi.org/10.1016/S0022-5347(01)61832-9|
|Abstract:||Purpose: To determine the effects of live BCG, autoclaved BCG and interferon α-2b on cytokine production in human bladder cancer cell lines. Materials and Methods: The release of nine cytokines from the human bladder cancer cell lines, RT4, RT112, SD, MGH and J82, was measured by ELISA assay. The mRNA level of IL-6 and GM-CSF was determined by RT-PCR. Results: BCG and/or interferon α-2b differentially increased IL-1β, IL-6, IL-8, GM-CSF and TNF-α production in the bladder cancer cells. High grade cell lines were more responsive to BCG whereas low grade lines were more sensitive to interferon α-2b. This correlated with cytotoxicity and growth inhibition induced by these agents. BCG could also induce low levels of IFN-α production in all the cell lines. Compared with live BCG, autoclaved BCG had no antiproliferative effect on MGH cells and was less effective in stimulating the production of IL-6, IL-8 and GM-CSF. However, autoclaved BCG was as effective as live BCG in inhibiting growth and stimulating IL-6 and TNF-α production of J82 cells. The combination of BCG and interferon α-2b also completely suppressed TGF-β1 production in the MGH and RT112 cell lines. Conclusions: The combination of BCG and interferon α-2b has additive effects in cytokine production from bladder cancer cells. This correlates with cytotoxicity and growth inhibition induced by these agents.|
|Source Title:||Journal of Urology|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 6, 2017
WEB OF SCIENCETM
checked on Nov 21, 2017
checked on Dec 17, 2017
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.