Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2005.07.029
Title: Rapid screening and characterisation of antioxidants of Cosmos caudatus using liquid chromatography coupled with mass spectrometry
Authors: Shui, G. 
Leong, L.P. 
Shih, P.W.
Keywords: Antioxidants
HPLC/MS
Phenolic compounds
Ulam raja
Issue Date: 2005
Source: Shui, G., Leong, L.P., Shih, P.W. (2005). Rapid screening and characterisation of antioxidants of Cosmos caudatus using liquid chromatography coupled with mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 827 (1) : 127-138. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2005.07.029
Abstract: Ulam raja (Cosmos caudatus) is used traditionally for improving blood circulation. In this study, it was found that ulam raja had extremely high antioxidant capacity of about 2400 mg l-ascorbic acid equivalent antioxidant capacity (AEAC) per 100 g of fresh sample. Antioxidant peaks in extract of ulam raja were firstly characterized using free radical spiking test through high performance liquid chromatography coupled with mass spectrometry (MS). Upon reaction with 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals, intensities of antioxidant peaks will be significantly reduced. HPLC/MSn was further applied to elucidate the chemical structures of antioxidant peaks characterized in the spiking test. More than twenty antioxidants were identified in ulam raja, and their chemical structures were proposed. The major antioxidants in ulam raja were attributed to a number of proanthocyanidins that existed as dimers through hexamers, quercetin glycosides, chlorogenic, neo-chlorogenic, crypto-chlorogenic acid and (+)-catching. High content of antioxidants antioxidants contained in ulam raja could be partly responsible for its ability to reduce oxidative stress. © 2005 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/28903
ISSN: 15700232
DOI: 10.1016/j.jchromb.2005.07.029
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