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|Title:||Expression of hepaCAM and Its Effect on Proliferation of Tumor Cells in Renal Cell Carcinoma|
|Citation:||Xun, C., Luo, C., Zhang, Q., Yan, L., Wu, X., Shen, S. (2010). Expression of hepaCAM and Its Effect on Proliferation of Tumor Cells in Renal Cell Carcinoma. Urology 75 (4) : 828-834. ScholarBank@NUS Repository. https://doi.org/10.1016/j.urology.2009.11.024|
|Abstract:||Objectives: To evaluate hepaCAM (hepatocyte cell adhesion molecule) gene expression in patients with renal cell carcinoma (RCC) and to explore its effect on proliferation of 786-0 cells. hepaCAM is a tumor suppressor gene, which has been identified as a member of immunoglobulin superfamily cell adhesion molecule. Methods: Two-step reverse transcription-polymerase chain reaction was used to determine hepaCAM expression in 30 paired (RCC and the adjacent non-RCC) renal specimens. Transfection studies were carried out by expressing green fluorescent protein and green fluorescent protein-fused hepaCAM in 786-0 cells. Results: Significant downregulation of hepaCAM was detected in 25 of 30 RCC patients tested. When transfected into 786-0 cells, the number of colony formation was reduced by 5-fold according to colony formation assay. MTT (3-diphenyltetrazolium bromide) showed the inhibition rates on the fourth, fifth, and sixth days of culturing were 26.5%, 38.1%, and 35.7%, respectively. Conclusion: Our data show that hepaCAM is frequently downregulated in RCC, and that exogenous hepaCAM exhibits antiproliferative effect on 786-0 cells, suggesting that silencing of hepaCAM may be associated with carcinogenesis of RCC. © 2010 Elsevier Inc. All rights reserved.|
|Appears in Collections:||Staff Publications|
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