Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2007.01.004
Title: Enhanced potency and efficacy of 29-mer shRNAs in inhibition of Enterovirus 71
Authors: Tan, E.L.
Chow, V.T.K. 
Poh, C.L. 
Tan, T.M.C.
Keywords: 29-mer shRNAs
Enhanced silencing effects
Enterovirus 71
Hand, foot and mouth disease (HFMD)
Potential antiviral therapy
RNA interference
Issue Date: 2007
Source: Tan, E.L., Chow, V.T.K., Poh, C.L., Tan, T.M.C. (2007). Enhanced potency and efficacy of 29-mer shRNAs in inhibition of Enterovirus 71. Antiviral Research 74 (1) : 9-15. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2007.01.004
Abstract: Enterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD) in young children. It has been associated with severe neurological complications and has caused significant mortalities in large-scale outbreaks in Asia. In this study, we demonstrated an enhanced silencing of EV71 through the use of chemically synthesized 29-mer shRNAs. The 29-mer shRNAs were designed to target three highly conserved regions of EV71 genome. Transfection of rhabdomyosarcoma (RD) cells with the 29-mer shRNAs significantly inhibited EV71 replication in a dose-dependent manner as demonstrated by reduction of viral RNA, VP1 protein and plaque forming units. The inhibitory effects were more potent and were achieved at 10-fold lower concentrations when compared to 19-mer siRNAs reported previously [Sim, A.C.N., Luhur, A., Tan, T.M.C., Chow, V.T.K., Poh, C.L., 2005. RNA interference against Enterovirus 71 infection. Virology 341, 72-79]. The viral inhibitory effects lasted 72 h post-infection and there was no adverse off-target silencing effect. Gene silencing by 29-mer shRNAs targeted at the 3Dpol region (sh-3D) was the most effective, achieving 91% viral inhibition. Further evaluation found that no enhanced inhibitory effects were observed when sh-3D was cotransfected with each of the other two candidates. This study showed an improvement in triggering RNAi using the more potent 29-mer shRNAs, indicating its therapeutic potential against EV71. © 2007 Elsevier B.V. All rights reserved.
Source Title: Antiviral Research
URI: http://scholarbank.nus.edu.sg/handle/10635/24667
ISSN: 01663542
DOI: 10.1016/j.antiviral.2007.01.004
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

20
checked on Apr 23, 2018

WEB OF SCIENCETM
Citations

18
checked on Apr 23, 2018

Page view(s)

249
checked on Apr 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.