Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23217
Title: Tissue engineering of a human periodontal ligament fibroblast membrane - Alveolar osteoblast scaffold double construct
Authors: CHOU AI MEI @ AMY CHOU
Keywords: Tissue engineering, periodontal ligament, alveolar osteoblast
Issue Date: 29-Aug-2007
Source: CHOU AI MEI @ AMY CHOU (2007-08-29). Tissue engineering of a human periodontal ligament fibroblast membrane - Alveolar osteoblast scaffold double construct. ScholarBank@NUS Repository.
Abstract: As a first step in the development of cell-scaffold constructs for periodontal regeneration, human periodontal ligament fibroblasts (hPDLF) and alveolar osteoblasts (hAO) were studied for their osteogenic potential. Both cell types produced varying levels of alkaline phosphatase and mineral-like tissue. Under culture expansion, hPDLF demonstrated a preservation of collagen-synthetic capability as seen from the synthesis of type I, III, V, XII and XIV. Subsequently, cell-substratum interaction of hPDLF on biocompatible and bioresorbable poly(e-caprolactone) (PCL) was examined. Cytocompatibility of alkali-treated PCL membranes was enhanced via a two-fold increase in cell adhesion rate, attributable to a greater accessibility of fibronectin cell-binding domain. Constructs consisting of perforated PCL membranes provided greater cell anchorage, and cell and matrix alignment than unperforated membranes, while retaining hPDLF phenotypic expression and promoting matrix maturation. hPDLF proliferated on alkali-treated, perforated PCL membranes, while hAO produced mineral-like tissue on alkali-treated PCL scaffolds at day 21. Vascularized, well-integrated hPDLF-hAO double construct was observed at day 28 of subcutaneous implantation in athymic mice, but no further osteogenesis in the earlier-mineralized matrix was seen.
URI: http://scholarbank.nus.edu.sg/handle/10635/23217
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
ChouAM.pdf8.64 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

203
checked on Jan 20, 2018

Download(s)

308
checked on Jan 20, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.