Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23140
Title: A novel mucosal absorption enhancer: 2-guanidyl chitosan
Authors: JIANG DAHAI
Keywords: guanidine, chitosan, absorption, enhancer, paracellular, transepithelial
Issue Date: 28-Mar-2007
Source: JIANG DAHAI (2007-03-28). A novel mucosal absorption enhancer: 2-guanidyl chitosan. ScholarBank@NUS Repository.
Abstract: Guanidyl chitosan derivatives were synthesized with aminoiminomethanesulfonic acid. The architecture of guanidino function in the final product was confirmed by elemental analysis, 13C-NMR spectrum and Sakaguchi color test. Five guanidyl chitosan derivatives with Mw ranging from 16.2 to 32.1 kDa, and degree of substitution (SD) ranging from 49 to 62% were prepared. In vitro cytotoxicity data suggest that the guanidylation of chitosan did not produce a more cytotoxic material by MTT assay. The effects of chitosan and guanidyl chitosan on the 14C-mannitol flux across the Caco-2 cell monolayers were evaluated at pH 5.5 and 7.4 at a concentration of 1 mg/ml. Guanidyl chitosan samples were able to promote the transepithelial 14C-mannitol flux at pH 5.5 and 7.4 while their chitosan peers failed. TEER measurements taken on the Caco-2 cell monolayers largely corroborated the 14C-mannitol transport data, and potential for application as an absorption enhancer in vitro.
URI: http://scholarbank.nus.edu.sg/handle/10635/23140
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
guanidyl chitosan.pdf923.02 kBAdobe PDF

OPEN

NoneView/Download

Page view(s)

245
checked on Dec 11, 2017

Download(s)

823
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.