Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23110
Title: Cloning, characterisation and functional analysis of horseshoe crab c-reactive proteins
Authors: TAN SEOK HWEE, SANDRA
Keywords: horseshoe crab C-reactive protein
Issue Date: 28-Apr-2005
Source: TAN SEOK HWEE, SANDRA (2005-04-28). Cloning, characterisation and functional analysis of horseshoe crab c-reactive proteins. ScholarBank@NUS Repository.
Abstract: The horseshoe crab, Carcinoscorpius rotundicauda, possesses a powerful innate immune system capable of clearing Gram-negative bacteria (GNB) infections that would be lethal to mice. Rapid bacterial clearance suggests the existence of frontline defense molecules that recognize lipopolysaccharide (LPS). C-reactive protein (CRP)-1 and -2 are extracellular lectins that bind LPS. This study aims to elucidate the roles of CRP-1 and -2. CRP-1 and-2 interact with different protein partners, and possibly mediate different immune response pathways. rCRP-1 and -2 interact with proteins of the na??ve cell-free hemolymph (CFH). This represents a pool of innate immune molecules that associate into a a??pathogen-recognition complexa?? early in infection. The interactions of both rCRP-1 and -2 are enhanced during infection and in the presence of calcium. CRP-2 exhibits bacterial agglutination and bactericidal activities. Calcium did not enhance bactericidal activity, suggesting it is not necessary for CRP-2a??s antimicrobial properties but is important for a??pathogen-recognition complexa?? recruitment.
URI: http://scholarbank.nus.edu.sg/handle/10635/23110
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
thesis_contents.pdf2.6 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

346
checked on Dec 11, 2017

Download(s)

227
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.