Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23107
Title: CCAAT/Enhancer binding protein alpha Knock-in mice exhibit early Liver glycogen storage, reduced susceptibility to hepatocellular carcinoma and increased hepatocyte functions
Authors: TAN EE HONG
Keywords: C/EBP alpha (C/EBPa); alpha fetoprotein (AFP), C/EBPa knock-in mice; glycogen; hepatocellular carcinoma; hepatocyte functions
Issue Date: 19-May-2007
Source: TAN EE HONG (2007-05-19). CCAAT/Enhancer binding protein alpha Knock-in mice exhibit early Liver glycogen storage, reduced susceptibility to hepatocellular carcinoma and increased hepatocyte functions. ScholarBank@NUS Repository.
Abstract: The CCAAT/enhancer binding protein alpha (C/EBPa) is a transcription factor that is highly expressed in the liver and adipocytes. C/EBPa is a regulator that transactivates the transcription of many energy-related genes. C/EBPa also induces cellular growth arrest and terminal differentiation in hepatocytes and adipocytes. Our lab has created a C/EBPa knock-in mouse model where a single-copy of C/EBPa gene was placed under the regulation of one of the two endogenous alleles of the mouse alpha fetoprotein (AFP) gene promoter. This strategic placement of the C/EBPa knock-in gene under the regulatory control of the AFP promoter allows for the artificial expression of the C/EBPa during fetal development and during hepatocarcinogenesis, when the AFP promoter is active. Taken together, the data show that C/EBPa knock-in mice exhibit early glycogen storage and glycogen synthase expression as compared to wild type mice. C/EBPa knock-in mice are also more resistant to hepatocarcinogenesis. C/EBPa knock-in hepatocytes also exhibit enhanced albumin production.
URI: http://scholarbank.nus.edu.sg/handle/10635/23107
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TanEH TABLE OF CONTENTS 01.pdf142.29 kBAdobe PDF

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TanEH INTRODUCTION 02.pdf749.72 kBAdobe PDF

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TanEH MATERIALS AND METHODS 03.pdf124.57 kBAdobe PDF

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TanEH RESULTS Part1 04.pdf1.46 MBAdobe PDF

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TanEH RESULTS Part2 05.pdf1.58 MBAdobe PDF

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TanEH DISCUSSION 06.pdf98.56 kBAdobe PDF

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TanEH REFERENCES 07.pdf131.96 kBAdobe PDF

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