Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/20922
Title: Extraction, determination and metabolic profiling of alkaloids in traditional Chinese medicines by modern analytical techniques
Authors: JIANG ZHANGJIAN
Keywords: Microwave-assisted extraction,pressurized hot water extraction,non-aqueous capillary electrophoresis,metabonomics,pyrrolizidine alkaloid,berberine
Issue Date: 14-Sep-2009
Source: JIANG ZHANGJIAN (2009-09-14). Extraction, determination and metabolic profiling of alkaloids in traditional Chinese medicines by modern analytical techniques. ScholarBank@NUS Repository.
Abstract: This dissertation is broadly divided into two parts. Part I focused on the isolation and determination of pyrrolizidine alkaloids (PAs) in traditional Chinese medicine with modern analytical techniques. Different methods of extraction such as microwave-assisted extraction and pressurized hot water extraction have been applied. The extraction efficiencies were compared with traditional method and analyzed by LC/MS. The effect of ion suppression in the botanical extracts was investigated. Concurrently, a non-aqueous capillary electrophoresis (NACE) technique with on-column preconcentration was developed for the separation and determination of PAs. Part II focused on the metabolic profiling study of the natural alkaloid, named berberine, in rats with GC/MS, LC/MS and 1H NMR. A new targeted data processing method for LC/MS was applied. The reliability of the new method was proven by compared with 1H NMR (non-targeted). The results obtained gave a further comprehensive picture of the metabolic changes induced by berberine in rat.
URI: http://scholarbank.nus.edu.sg/handle/10635/20922
Appears in Collections:Ph.D Theses (Open)

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