Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17026
Title: Role of human growth hormone-regulated HOXA1 in mammary gland neoplastic progression
Authors: ZHANG XIN
Keywords: mammary gland neoplasia, GH, HOXA1, Bcl-2, E-cadherin, Rac1
Issue Date: 5-Aug-2005
Source: ZHANG XIN (2005-08-05). Role of human growth hormone-regulated HOXA1 in mammary gland neoplastic progression. ScholarBank@NUS Repository.
Abstract: Abstract Name: Zhang Xin Degree: Ph.D. Dept: Medicine Thesis title: Role of human growth hormone-regulated HOXA1 in mammary gland neoplastic progression Previous microarray studies showed that autocrine human growth hormone (hGH) production by human mammary carcinoma cells (MCF-7) upregulated the homeobox gene, HOXA1. The present work investigated the role of autocrine GH regulated HOXA1 in MCF-7 and MCF-10A (immortalized but not transformed) cell lines in vitro, and in severe combined immunodeficient (SCID) mice in vivo. Forced expression of HOXA1 in stably transfected MCF-7 cells resulted in increased total cell number primarily by the promotion of cell survival mediated by the upregulation of Bcl-2, and enhancement of anchorage independent proliferation and colony formation in soft agar. Furthermore, forced expression of HOXA1 was sufficient to result in the oncogenic transformation of MCF-10A with aggressive in vivo tumor formation in SCID mice. Finally, HOXA1 expression was found to be significantly increased in MCF-7 cells at full confluence and this increment was demonstrated to be directed by E-cadherin signaling through Rac1.
URI: http://scholarbank.nus.edu.sg/handle/10635/17026
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