Effects of grape seed extract and calorie restriction on aging process

Abstract

We hypothesized that grape seed extract (GSE) and calorie restriction (CR) attenuated age related oxidative damage in middle-aged rats. Firstly, real time RT-PCR with valid housekeeping gene protocol was established. We found the significantly decreased catalase and Cu/Zn-SOD expression in rat kidney aging. Secondly, the effect of GSE and CR in middle-aged rats was investigated. The low and high dose GSE and CR significantly decreased urinary 8-isoprostane (P<0.05) and the high dose GSE and CR have significantly decreased protein carbonyl in kidney (P<0.05).The related molecular mechanism was explored by microarray and real time RT-PCR. Our data indicated that GSE could prevent age related oxidative damages which might be regulated through the decreased 15-LO and S100A8 gene expression. CR retarded age related oxidative damages and renal pathological changes, which might be regulated through the increased catalase, Mn-SOD and kallikrein and the reduced expression of C3 and CCL-5 gene expression.