Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16638
Title: An advanced biohybrid nano-microscaffold for tendon/ligament tissue engineering
Authors: SAMBIT SAHOO
Keywords: Tissue Engineering, Tendon and Ligament, Biomimetic Scaffolds, Electrospinning, Bone Marrow Stromal Cells
Issue Date: 9-Feb-2009
Source: SAMBIT SAHOO (2009-02-09). An advanced biohybrid nano-microscaffold for tendon/ligament tissue engineering. ScholarBank@NUS Repository.
Abstract: Fibre-based scaffolds have been widely used in tendon/ligament tissue engineering; however there is still a need for a scaffold that provides suitable mechanical properties along with biological signals required for tendon/ligament regeneration from stem cells. This PhD project developed a novel biodegradable nano-microfibrous polymer scaffold system by electrospinning nanofibers onto a knitted microfibrous scaffold. A silk-based scaffold was developed and optimized to improve the mechanical/degradation properties for ligament/tendon repair. Polymeric nanoscaffolds capable of sustained release of bioactive growth factor bFGF were fabricated using modifications of the electrospinning technique - blend electrospinning and coaxial electrospinning. Biohybrid nano-microscaffolds were fabricated by coating blend electrospun bFGF-releasing nanofibres over the optimised knitted silk scaffolds. The novel scaffold system not only facilitated cell seeding and promoted cell proliferation, but also stimulated BMSC differentiation into a fibroblastic lineage, generating a tendon/ligament analogue that has the potential to be used to repair an injured tendon/ligament.
URI: http://scholarbank.nus.edu.sg/handle/10635/16638
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Sambit Sahoo_PhD_BIE_HT040380N_2008.pdf13.31 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

339
checked on Dec 11, 2017

Download(s)

297
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.