Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16593
Title: GENOME-WIDE SCREEN OF GENES THAT REGULATE LIPID DROPLET DYNAMICS IN SACCHAROMYCES CEREVISIAE
Authors: FEI WEIHUA
Keywords: Lipid droplets; Yeast; Genome-wide Screen; Fld1; Seipin
Issue Date: 7-Jan-2009
Source: FEI WEIHUA (2009-01-07). GENOME-WIDE SCREEN OF GENES THAT REGULATE LIPID DROPLET DYNAMICS IN SACCHAROMYCES CEREVISIAE. ScholarBank@NUS Repository.
Abstract: Lipid droplets consist of a highly hydrophobic core of neutral lipids and are surrounded by a monolayer of phospholipids. They are associated with many devastating diseases. Several mammalian proteins have been shown to have an important role in lipid droplet biosynthesis, but many remain unidentified. In an effort to identify genes that regulate lipid droplet dynamics, I screened the entire collection of viable single-gene deletion yeast strains, and found 16 mutants with markedly reduced accumulation of lipid droplets and 117 mutants with increased accumulation of lipid droplets. Remarkably, cells lacking Ylr404wp, an ortholog of human lipodystrophy protein seipin, synthesized supersized lipid droplets. Moreover, lipid droplets of ylr404w cells demonstrated enhanced fusion both in vivo and in vitro. Phospholipid profiling showed that a shift from long-chain to medium/short-chain fatty acid incorporation into phospholipids occurs due to YLR404W deletion. This result may suggest that modulation of the synthesis of phospholipids is critical for lipid droplet assembly as well as adipogenesis.
URI: http://scholarbank.nus.edu.sg/handle/10635/16593
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Fld1 LD fusion.wmv200.81 kBWMV

OPEN

None Preview online
View/Download
THESIS.pdf4.49 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

322
checked on Dec 11, 2017

Download(s)

48
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.