Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15888
Title: Carbon nanotubes and branched-DNA based nucleic acid assays: Towards a PCR-free detection and quantification of nucleic acids
Authors: LEE AI CHENG
Keywords: CNT PCR Branched-DNA BCR-ABL Biosensor Electrochemical
Issue Date: 22-May-2009
Source: LEE AI CHENG (2009-05-22). Carbon nanotubes and branched-DNA based nucleic acid assays: Towards a PCR-free detection and quantification of nucleic acids. ScholarBank@NUS Repository.
Abstract: This research is directed towards the development of PCR-free ultrasensitive nucleic acids detection based on signal amplification approach. The solution holds promise for simple and low cost nucleic acids detection in portable devices, point-of-care and early disease diagnostic applications. Two types of nucleic acid assays based on novel carbon nanotube (CNT)-based label and branched-DNA (bDNA) amplifier were developed for the detection of a human acute lymphocytic leukaemia (ALL) oncogene, namely, p185 BCR-ABL fusion transcript. These assays have been successfully validated for PCR-free detection and quantification of p185 BCR-ABL in the samples of mRNA population extracted from positive ALL cell line SUP-B15, in comparison to those obtained from negative cell lines HL-60. Without the PCR amplification, the electrochemical nucleic acids assays utilizing CNT-based label and bDNA amplifier enabled direct detection of p185 BCR-ABL in as little as 65 ng and 4.6 ng of mRNA, respectively.
URI: http://scholarbank.nus.edu.sg/handle/10635/15888
Appears in Collections:Ph.D Theses (Open)

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