Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15527
Title: Study the Anticancer Activity of Bioactive Peptide Cecropins A Class of Bioactive peptides, Using Electrophysiological Methods
Authors: ZHAO LIPING
Keywords: Cecropin B; Anticancer Peptide; Selectivity; Pore Formation; Patch Clamp
Issue Date: 18-Aug-2006
Source: ZHAO LIPING (2006-08-18). Study the Anticancer Activity of Bioactive Peptide Cecropins A Class of Bioactive peptides, Using Electrophysiological Methods. ScholarBank@NUS Repository.
Abstract: Cecropin peptides possess antibacterial and anticancer activity making them of great therapeutic value. The general mechanism of cecropins' actions is thought to disrupt cell membranes through direct membrane-peptide interactions by studies on artificial membrane systems. We used cell-attached patch clamp to study the effects of cecropin B and its analog cecropin B-3 on different cell lines (HeLa and Hek293), showing that the tumor cell line HeLa was sensitive to CB by the formation of channel-like pores on the patch in contrast to the unsusceptible non-tumor cell line Hek293. A comparison of surface charge and surface morphology of the two cells gave us some clues to address their different responses. The structure-function relationship of cecropin peptides at the single cell level was also discussed. Finally, in order to improve the throughput of current patch clamp method, a patch clamp chip prototype based on MEMS has been proposed and characterized.
URI: http://scholarbank.nus.edu.sg/handle/10635/15527
Appears in Collections:Master's Theses (Open)

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