Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15096
Title: Identification and characterization of agrobacterium tumefaciens virD2-binding protein
Authors: GUO MINLIANG
Keywords: Agrobacterium tumefaciens, VirD2-binding protein, T-DNA transfer, Plasmid conjugation, Type IV secretion system, Pull-down assay
Issue Date: 16-Dec-2005
Source: GUO MINLIANG (2005-12-16). Identification and characterization of agrobacterium tumefaciens virD2-binding protein. ScholarBank@NUS Repository.
Abstract: In this thesis, a novel A. tumefaciens VirD2-binding protein (VBP) was identified. Homologous search showed that A. tumefaciens has two additional genes highly homologous to the identified vbp gene. So, A. tumefaciens contains the redundancy of three homologs for the VirD2-binding protein. Mutations of three vbp genes showed that VBP proteins were involved in the tumorigenesis of A. tumefaciens. Pull-down and co-immunoprecipitation results showed that VBP proteins could bind both VirD2 and some components of VirB/D4 type IV secretion system (T4SS). The C-terminal sequence of VBP proteins possess an important feature of the known T4SS substrates, indicating that VBP proteins may help T-complex to be recruited to VirB/D4 transport apparatus. VBP proteins were also shown to be involved in some plasmid conjugation system. Therefore, VBP proteins were defined as a new class of proteins; recruiting proteins that can recruit the cytoplasmic DNA transfer intermediate to the membrane transport apparatus.
URI: http://scholarbank.nus.edu.sg/handle/10635/15096
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Guo M L.pdf2.05 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

299
checked on Dec 18, 2017

Download(s)

341
checked on Dec 18, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.