Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/15087
Title: Ohanin - A novel protein from king cobra (Ophiophagus hannah) venom
Authors: PUNG YUH FEN
Keywords: ohanin, hypolocomotion, hyperalgesia, B30.2-like domain, PRY-SPRY domains, vespryns
Issue Date: 15-Dec-2005
Source: PUNG YUH FEN (2005-12-15). Ohanin - A novel protein from king cobra (Ophiophagus hannah) venom. ScholarBank@NUS Repository.
Abstract: We describe the identification, purification, and structural and functional characterization of the first member of a new family of snake venom proteins. The novel protein, named ohanin, was identified by LC/MS, purified by a two-step chromatography, and sequenced using Edman degradation sequencing. It shows 44 % identity to PRY-SPRY domains of B30.2 domain-containing proteins. Ohanin lowers the locomotor activity and induces hyperalgesia in mice when administered by both intraperitoneal and intracerebroventricular routes. Both these pharmacological effects are mediated through its effect on the central nervous system. As ohanin is found in low abundance in king cobra venom, we have designed and constructed a synthetic gene for ohanin. The recombinant protein expressed in E. coli is structurally and functionally similar to the native protein. In addition, we have also cloned and sequenced the cDNA for ohanin. Its full-length cDNA sequence of 1558 bp encodes for prepro-ohanin with a propeptide segment at the C-terminal. Recombinant pro-ohanin shows potent effect on locomotion when injected i. c. v. but not when injected i. p. Thus maturation appears to be crucial for the biological activity of ohanin. The genomic DNA sequencing indicates the presence of five exons and four introns. Interestingly, the second exon encoding the 5a??-untranslated region is alternatively spliced. All these findings together indicate that ohanin forms a new subfamily of B30.2 domain-containing proteins.
URI: http://scholarbank.nus.edu.sg/handle/10635/15087
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Thesis cover n spine.pdf6.59 kBAdobe PDF

OPEN

NoneView/Download
Thesis front colour.pdf9.85 kBAdobe PDF

OPEN

NoneView/Download
201203_front.pdf66.94 kBAdobe PDF

OPEN

NoneView/Download
Combine12345_02082005_final.pdf2.62 MBAdobe PDF

OPEN

NoneView/Download
Appendices.pdf144.01 kBAdobe PDF

OPEN

NoneView/Download

Page view(s)

370
checked on Jan 21, 2018

Download(s)

2,983
checked on Jan 21, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.