The myelination effects of low level laser therapy on the rat's sciatic nerve, following lysolecithin-induced demyelination

Abstract

Low-level Laser therapy (LLLT) has been shown to have beneficial effects on pain reduction and nerve regeneration. But, its effects particularly on myelin sheath regeneration have not been well established. Myelin sheath is an insulating covering surrounding the nerve fibers. It is essential for proper conduction of nerve impulses. Demyelination leads to transmission of impulses to adjacent fibers producing neuropathic pain. Peripheral demyelination can be produced by topical application of lysophosphatidyl choline on sciatic nerve of a rat model without axonal loss. An experimental group would receive LLLT as compared to the control. The morphological changes in the myelin sheath are to be determined using electron microscopy. G-ratios (axon diameter: fiber diameter) would also be calculated to determine the relative myelin thickness following LLLT.