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Title: | THE APPLICATION AND OPTIMISATION OF BIO-AUGMENTATION IN THE ANAEROBIC DIGESTION OF CELLULOSIC SUBSTRATES | Authors: | JONATHAN LEE TIAN EN | ORCID iD: | orcid.org/0000-0002-6976-1831 | Keywords: | anaerobic digestion, bioaugmentation, cellulosic substrates, biogas, methane, LCA | Issue Date: | 11-Jun-2018 | Citation: | JONATHAN LEE TIAN EN (2018-06-11). THE APPLICATION AND OPTIMISATION OF BIO-AUGMENTATION IN THE ANAEROBIC DIGESTION OF CELLULOSIC SUBSTRATES. ScholarBank@NUS Repository. | Abstract: | Anaerobic digestion of cellulosic substrates in Singapore was assessed via a Life Cycle Analysis against three other waste management methods and found to be environmentally friendly in terms of greenhouse gas emissions and energy produced. An acclimatisation was carried out on cowgrass to obtain a microbial community adapted for digesting grass well, before perturbing said community. The community structures were compared with traditional molecular method DGGE and NGS Illumina and two microbes were found to be missing in the maladapted community. Bioaugmentation was carried out for grass, with microbes from the hydrolytic, acetogenic and methanogenic processes of the multi-step anaerobic digestion process. A triple augmentation consisting of microbes from all steps worked best. Thereafter an optimisation of bioaugmentation was carried out utilizing Taguchi’s method and the optimal combination of Clostridium cellulolyticum, Mesotoga infera and Methanosaeta concilii was elucidated. This combination was validated in a semi-continuous scaled-up setup. | URI: | http://scholarbank.nus.edu.sg/handle/10635/148543 |
Appears in Collections: | Ph.D Theses (Open) |
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