Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14348
Title: Functional characterisation of a novel nuclear protein, Hcc-1
Authors: LEAW CHUI LI
Keywords: DEAD-box family , Proliferation inhibition, SAP DNA binding domain, Scaffold/ matrix attachment region DNA
Issue Date: 8-Nov-2004
Source: LEAW CHUI LI (2004-11-08). Functional characterisation of a novel nuclear protein, Hcc-1. ScholarBank@NUS Repository.
Abstract: Hcc-1 is a novel nuclear protein containing the SAP DNA binding domain. We have shown that Hcc-1 binds to both double-stranded and single-stranded DNA with higher affinity for single-stranded DNA. In addition, it is also shown to bind specifically to scaffold/matrix attachment region (S/MAR) DNA. This nucleic acid binding characteristic suggests a potential function of Hcc-1 as a heterogeneous ribonucleoprotein complex. Using a yeast two-hybrid screen, we have identified two DEAD-box RNA helicase proteins, BAT1 and DDX39, as interacting proteins with Hcc-1. The interactions with these RNA helicases point to a function of Hcc-1 in RNA biogenesis such as the spliceosome assembly. Furthermore, overexpression of Hcc-1 in HEK293 cell line showed a significant slower growth rate (p = 0.0173 compared to controls) and accumulations of cells (p = 0.0276 compared to HEK293 cells) at the G2/M phase of the cell cycle. Interestingly, the interacting protein, BAT1 was also implicated in growth inhibition. Taken together, these findings propose potential roles for Hcc-1 in growth regulation and RNA metabolism.
URI: http://scholarbank.nus.edu.sg/handle/10635/14348
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
01-Chap.pdf89.71 kBAdobe PDF

OPEN

NoneView/Download
02-Chapter 1 Introduction.pdf40.02 kBAdobe PDF

OPEN

NoneView/Download
03-flow chart of Hcc-1.pdf12.11 kBAdobe PDF

OPEN

NoneView/Download
04-Chapter 2 Protein-nucleic acid interaction.pdf139.66 kBAdobe PDF

OPEN

NoneView/Download
05-Materials and methods Chapter 2.pdf109.92 kBAdobe PDF

OPEN

NoneView/Download
06-Results chapter 2.pdf955.79 kBAdobe PDF

OPEN

NoneView/Download
07-Chapter 3 Protein-protein interaction.pdf58.08 kBAdobe PDF

OPEN

NoneView/Download
08-Materials and methods Chapter 3.pdf99.97 kBAdobe PDF

OPEN

NoneView/Download
09-Results chapter 3.pdf1.17 MBAdobe PDF

OPEN

NoneView/Download
10-Chapter 4 Cell proliferation study.pdf29.62 kBAdobe PDF

OPEN

NoneView/Download
11-Materials and methods Chapter 4.pdf66.49 kBAdobe PDF

OPEN

NoneView/Download
12-Results chapter 4.pdf163.66 kBAdobe PDF

OPEN

NoneView/Download
13-Chapter 5 concluding remarks and future prospects.pdf23.56 kBAdobe PDF

OPEN

NoneView/Download
14-References.pdf71.31 kBAdobe PDF

OPEN

NoneView/Download
15-Appendix A & B.pdf74.36 kBAdobe PDF

OPEN

NoneView/Download

Page view(s)

228
checked on Dec 11, 2017

Download(s)

3,549
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.