Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/138676
Title: OPTICAL MANIPULATIONS OF C.ELEGANS BIO ORGANISMS IN MICROFLUIDIC CHIPS FOR BEHAVIOURAL STUDIES
Authors: ISSENJOU ACHILLE MICHEL JACQUES
Keywords: Caenorhabditis elegans, optogenetic, laser behavioural experiments, microfluidic chip, UV soft lithography, spatial light modulator
Issue Date: 12-Oct-2017
Citation: ISSENJOU ACHILLE MICHEL JACQUES (2017-10-12). OPTICAL MANIPULATIONS OF C.ELEGANS BIO ORGANISMS IN MICROFLUIDIC CHIPS FOR BEHAVIOURAL STUDIES. ScholarBank@NUS Repository.
Abstract: Caenorhabditis elegans is a 1mm genetically modifiable roundworm. Here, we study a strain of modified optogenetic C.elegans, more sensitive to blue light, and analyse its reaction to 473nm, 532nm, 650nm and 1064nm lasers at different powers for behavioural experiments inside a bio-compatible PDMS microfluidic chip. Three generations of chips were created to replicate the worms natural environment. They have been fabricated by adapting a UV soft lithography procedure. Most notably, the optogenetic strain had an instantaneous aversive reaction after exposure to low power blue beams. Both modified and non-modified strains reacted to the heat produced by infrared laser, as confirmed with a thermo-sensitive dye. Finally, we built a complex optical set-up, containing a spatial light modulator diffracting the blue laser beam into various patterns projected onto the chips, allowing to control worms motion. Coupled with infrared laser punishment, this could allow biologists to perform numerous behavioural experiments on C.elegans.
URI: http://scholarbank.nus.edu.sg/handle/10635/138676
Appears in Collections:Master's Theses (Open)

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