Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13657
Title: Cloning and characterization of Blo t 11, a high molecular weight Blomia tropicalis allergen homologous to paramyosin
Authors: JOHN DONNIE A RAMOS
Keywords: Allergen, Blo t 11, Blomia tropicalis, house dust mite, paramyosin, recombinant protein
Issue Date: 5-Feb-2004
Source: JOHN DONNIE A RAMOS (2004-02-05). Cloning and characterization of Blo t 11, a high molecular weight Blomia tropicalis allergen homologous to paramyosin. ScholarBank@NUS Repository.
Abstract: Three polymorphic variants of Blo t 11 were isolated and characterized. Recombinant Blo t 11 significantly reacted to 52% of IgE from allergic patients. Although multiple IgE and IgG epitopes were mapped throughout the molecule, an immunodominant region (fD) was delineated and characterized. The native Blo t 11, isolated by immunoaffinity purification and confirmed by MALDI-TOF MS, was shown to exist in at least 10 isoforms. Comparative in vitro and in vivo studies between the native and recombinant Blo t 11 showed that fD has comparable IgE reactivity with its native counterpart. Blo t 11 monoclonal antibodies, generated by DNA immunization, were exploited fully for detection, quantitation and isolation of native and recombinant Blo t 11. Furthermore, four Blo t 11 hybridoma clones with mutations on immunoglobulin constant region were characterized.
URI: http://scholarbank.nus.edu.sg/handle/10635/13657
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