Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/136019
Title: THE NON-STRUCTURAL ROLES OF DENGUE VIRUS STRUCTURAL CAPSID PROTEIN
Authors: CHONG MUN KEAT
Keywords: dengue virus, capsid protein, structural protein, non-structural roles, nuclear localization, cell cycle
Issue Date: 21-May-2013
Source: CHONG MUN KEAT (2013-05-21). THE NON-STRUCTURAL ROLES OF DENGUE VIRUS STRUCTURAL CAPSID PROTEIN. ScholarBank@NUS Repository.
Abstract: Dengue virus (DENV) is the most important mosquito-borne viral disease. This study unraveled the nuclear translocation mechanism of DENV capsid (C) protein and its non-structural roles in the nucleus. Site-directed mutagenesis studies found that second bipartite nuclear localization signal motif was the most essential domain for nuclear localization. It was shown for the first time that DENVC protein interacted with importin-a protein which served as a bridge for importin-ß protein to bring the whole complex into the nucleus. To identify possible roles of C protein in the nucleus, purified biotinylated full-length C protein was used to screen for novel interacting partners via ProtoArray® technology-based protein microarray. The list of potential interacting partners suggested that DENVC may be involved in cell cycle control, apoptosis, and hijacking host cell transcriptional and translational machinery to favor the virus replication. This study allows better understanding of the roles of nuclear phase during DENV infection.
URI: http://scholarbank.nus.edu.sg/handle/10635/136019
Appears in Collections:Ph.D Theses (Open)

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