Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/135192
Title: INVESTIGATION OF TGF-??ASSOCIATED MASTER-LIKE TRANSCRIPTION FACTORS IN BREAST CANCER
Authors: ZHANG SITING
Keywords: TGF-β signaling, Breast cancer, Master transcription factor, E2F1, Super-enhancer, ChIP-seq
Issue Date: 21-Feb-2017
Source: ZHANG SITING (2017-02-21). INVESTIGATION OF TGF-??ASSOCIATED MASTER-LIKE TRANSCRIPTION FACTORS IN BREAST CANCER. ScholarBank@NUS Repository.
Abstract: Transforming growth factor β (TGF-β) signaling is important in early development, lineage specification, and tumorigenesis. In cancer, TGF-β can function as either a tumor suppressor or a tumor promoter at different stages of carcinogenesis. In early development, the context specificity of TGF-β signaling is partially determined by master transcription factors guiding SMAD-dependent genomic regulation. We hypothesize that a similar role for “master-like transcription factors” may determine context-specific genomic regulation of TGF-β signaling in tumorigenesis. We address this problem through integrative genomic studies and bioinformatics analysis of TGF-β-responsive breast cancer cell models, as well as data from TCGA. We identify a context-specific interaction between E2F Transcription Factor 1 (E2F1) and SMAD-dependent signaling in MCF7, an ER-positive luminal-A breast cancer model. E2F1 is a potential master-like transcription factor in this setting: it is not required for SMAD3 binding, but co-occupies genomic sites with SMAD3 and coordinates with SMAD3 to regulate downstream genes. Moreover, 138 putative super-enhancers were found in response to TGF-β in MCF7, whose associated genes were enriched in ten oncogenic gene sets including RB-mediated oncogenes and TGF-β mediated oncogenes.
URI: http://scholarbank.nus.edu.sg/handle/10635/135192
Appears in Collections:Ph.D Theses (Restricted)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
PhD Thesis_Zhang Siting_A0095170H.pdf3.83 MBAdobe PDF

RESTRICTED

NoneLog In

Page view(s)

33
checked on Jan 20, 2018

Download(s)

15
checked on Jan 20, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.