Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/134686
Title: DROPLET MICROFLUIDIC APPROACHES FOR SINGLE-CELL PROTEOLYTIC FUNCTIONAL ASSAY
Authors: JING TENGYANG
Keywords: Droplet Microfluidics, Single-Cell Analysis, Proteolytic Functional Assay
Issue Date: 29-Jul-2016
Source: JING TENGYANG (2016-07-29). DROPLET MICROFLUIDIC APPROACHES FOR SINGLE-CELL PROTEOLYTIC FUNCTIONAL ASSAY. ScholarBank@NUS Repository.
Abstract: Every cell has its distinct phenotype, behavior and function. However, conventional biological analytical assays, such as western blot and enzyme-linked immunosorbent assay, adopt ensemble measurements to extract the average information from non-homogeneous cell populations without taking into account the critical details of individual cells. Such simplistic methods could generate misleading results from highly heterogeneous cells, like cancer and immune cells. In order to accurately and effectively study functional heterogeneity of single cancer and immune cells, we developed a series of microfluidic droplet platforms. We first developed a novel and label-free droplet system to enhance single-cell encapsulation efficiency by incorporating jetting and size-based sorting. Next, we applied the droplet microfluidics to study single-cell matrix metalloproteinase activity of various breast cancer cell lines and patient-derived circulating tumor cells. Lastly, we developed a new droplet microfluidic system to study matrix metalloproteinase of single leukocytes with solving the pre-encapsulation contamination issue.
URI: http://scholarbank.nus.edu.sg/handle/10635/134686
Appears in Collections:Ph.D Theses (Open)

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