Please use this identifier to cite or link to this item:
|Title:||Rice (Oryza sativa) centromeric regions consist of complex DNA|
|Citation:||Dong, F., Miller, J.T., Jackson, S.A., Wang, G.-L., Ronald, P.C., Jiang, J. (1998-07-07). Rice (Oryza sativa) centromeric regions consist of complex DNA. Proceedings of the National Academy of Sciences of the United States of America 95 (14) : 8135-8140. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.95.14.8135|
|Abstract:||Rice bacterial artificial chromosome clones containing centromeric DNA were isolated by using a DNA sequence (pSau3A9) that is present in the centromeres of Gramineae species. Seven distinct repetitive DNA elements were isolated from a 75-kilobase rice bacterial artificial chromosome clone. All seven DNA elements are present in every rice centromere as demonstrated by fluorescence in situ hybridization. Six of the elements are middle repetitive, and their copy numbers range from ≃50 to ≃300 in the rice genome. Five of these six middle repetitive DNA elements are present in all of the Gramineae species, and the other element is detected only in species within the Bambusoideae subfamily of Gramineae. All six middle repetitive DNA elements are dispersed in the centromeric regions. The seventh element, the RCS2 family, is a tandem repeat of a 168-bp sequence that is represented ≃6,000 times in the rice genome and is detected only in Oryza species. Fiber-fluorescence in situ hybridization analysis revealed that the RCS2 family is organized into long uninterrupted arrays and resembles previously reported tandem repeats located in the Centromeres of human and Arabidopsis thaliana chromosomes. We characterized a large DNA fragment derived from a plant centromere and demonstrated that rice centromeres consist of complex DNA, including both highly and middle repetitive DNA sequences.|
|Source Title:||Proceedings of the National Academy of Sciences of the United States of America|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 17, 2019
WEB OF SCIENCETM
checked on Jan 9, 2019
checked on Dec 14, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.