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https://doi.org/10.1097/00042737-199807000-00008
Title: | Validation of [13C]urea breath test for Helicobacter pylori using a simple gas chromatograph-mass selective detector | Authors: | Lee, H.-S. Gwee, K.A. Teng, L.-Y. Kang, J.-Y. Yeoh, K.-G. Wee, A. Chua, B.-C. |
Keywords: | [13C]urea breath test Gas chromatograph-mass selective detector Helicobactor pylori Isotope ratio analysis |
Issue Date: | Jul-1998 | Citation: | Lee, H.-S., Gwee, K.A., Teng, L.-Y., Kang, J.-Y., Yeoh, K.-G., Wee, A., Chua, B.-C. (1998-07). Validation of [13C]urea breath test for Helicobacter pylori using a simple gas chromatograph-mass selective detector. European Journal of Gastroenterology and Hepatology 10 (7) : 569-572. ScholarBank@NUS Repository. https://doi.org/10.1097/00042737-199807000-00008 | Abstract: | Objective. Isotope ratio mass spectrometry (IRMS) is the accepted method for accurately measuring the 13CO2:12CO2 ratio in the non-invasive and non-readioactive [13C]urea breath test (13C-UBT) for Helicobactor pylori. The IRMS instrument, an expensive and highly specialized analyser, is rarely available. The objective of this project was to modify and validate the use of a simple bench-top gas chromatograph-mass selective detector (GC-MSD) for 13C-UBT. Methods. Breath samples from 71 patients were taken at baseline and 30 min after ingestion of 100 mg [13C]urea. The breath samples were analysed using GC-MSD in the selected ion monitoring mode. The reference 13CO2:12CO2 ratio was from NBS19 obtained from the US National Institute of Standards and Technology. 13CO2:12CO2 ratios of the breath samples were determined. Excess delta per thousand (per mil, δ‰) of the 30 min sample over the baseline (Δδ‰) of ≤ 6Δδ‰ was considered H. pylori positive. Results from 13C-UBT and histology determined blind to each other were compared. Results. The coefficient of variation of the reference 13CO2:12CO2 ratio was 0.06%. Using histology as the 'gold standard', the sensitivity (97.9%) and specificity (95.8%) of the GC-MSD 13C-UBT were comparable to those of other methods of H. pylori diagnosis. Conclusion. A gas chromatograph coupled to a mass selective detector that is available in many analytical and biomedical laboratories can be used for the 13C-UBT. This method will increase the availability and reduce the cost of this non-invasive, non-radioactive diagnostic test. | Source Title: | European Journal of Gastroenterology and Hepatology | URI: | http://scholarbank.nus.edu.sg/handle/10635/133992 | ISSN: | 0954691X | DOI: | 10.1097/00042737-199807000-00008 |
Appears in Collections: | Staff Publications |
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