Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00432-008-0508-x
Title: Frequent inactivation of RUNX3 by promoter hypermethylation and protein mislocalization in oral squamous cell carcinomas
Authors: Gao, F.
Huang, C.
Lin, M.
Wang, Z.
Shen, J.
Zhang, H. 
Jiang, L.
Chen, Q.
Keywords: Hypermethylation
Methylation-specific PCR
Oral squamous cell carcinoma
Protein mislocalization
Runt-related transcription factor 3
Issue Date: May-2009
Source: Gao, F., Huang, C., Lin, M., Wang, Z., Shen, J., Zhang, H., Jiang, L., Chen, Q. (2009-05). Frequent inactivation of RUNX3 by promoter hypermethylation and protein mislocalization in oral squamous cell carcinomas. Journal of Cancer Research and Clinical Oncology 135 (5) : 739-747. ScholarBank@NUS Repository. https://doi.org/10.1007/s00432-008-0508-x
Abstract: Purpose: RUNX3 is a functionally important component in transforming growth factor-beta (TGF-β) mediated signaling pathway. Epigenetic silencing expression of RUNX3, as well as aberrant cytoplasmic retention of RUNX3 protein are causely involved in gastric carcinogenesis. Here, we examined the expression of RUNX3 gene and protein in oral squamous cell carcinomas (OSCCs) and analyzed the methylation status of RUNX3 promoter region. Methods: About 10 normal oral mucosa and 30 OSCCs were collected to examine RUNX3 expression by RT-PCR analysis and immunohistochemistry assay using anti-RUNX3 monoclonal antibody R3-6E9. Methylation-specific PCR was carried out on the same specimens to analyze the methylation status of RUNX3 promoter. In addition, the stored paraffin-embedded specimens, including 40 oral leucoplakia (OLK) and 120 OSCCs, were examined by immunohistochemistry assay. Results: RUNX3 gene and protein were underexpressed in OSCCs due to promoter hypermethylation. Protein mislocalization occured frequently. Both downregulation of RUNX3 protein expression (P = 0.001) and protein mislocalization (P = 0.001) were correlated with the differentiation grades in OSCCs. Conclusions: RUNX3 plays an important role in oral carcinogenesis. It may be a useful diagnostic marker and a potential therapeutic target for OSCC. © 2008 Springer-Verlag.
Source Title: Journal of Cancer Research and Clinical Oncology
URI: http://scholarbank.nus.edu.sg/handle/10635/133359
ISSN: 01715216
DOI: 10.1007/s00432-008-0508-x
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