Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/133026
Title: Granulocyte-macrophage colony-stimulating factor overrides the immunosuppressive function of corticosteroids on rat pulmonary dendritic cells
Authors: Lim, T.K. 
Chen, G.-H.
McDonald, R.A.
Toews, G.B.
Keywords: Antigen presentation
Co- stimulation
Dexamethasone
GM-CSF
Pulmonary dendritic cells
Issue Date: 1996
Source: Lim, T.K., Chen, G.-H., McDonald, R.A., Toews, G.B. (1996). Granulocyte-macrophage colony-stimulating factor overrides the immunosuppressive function of corticosteroids on rat pulmonary dendritic cells. Stem Cells 14 (3) : 292-299. ScholarBank@NUS Repository.
Abstract: Pulmonary dendritic cells (DC) are present in extremely small numbers, but they are the most potent antigen-presenting cells in the lungs. Pure populations of DC can be isolated from the lung following collagen digestion, Percoll gradient centrifugation, removal of phagocytic cells and flow cytometric sorting for cells which exhibit high levels of surface major histocompatibility complex (MHC) class II molecules. Exogenous GM-CSF enhances this immunostimulatory capacity of the pulmonary DC. Soluble factors produced by type H airway epithelial cells and interstitial macrophages also enhance the immunostimulating capacity of pulmonary DC while alveolar macrophages suppress it. Thus, the function of DC may be regulated by locally produced cytokines. Corticosteroids are widely used as immunosuppressive agents in pharmacotherapy. While these agents are known to inhibit T cell proliferation and macrophage activation, their effects on DC are not known. We found that dexamethasone (Dex) pretreatment resulted in about a 50% reduction in the immunostimulatory capacity of rat pulmonary DC. This was associated with downregulation of MHC class II (Ia) expression. Dex-induced suppression of DC function could be restored with GM-CSF. We conclude that corticosteroids downregulate antigen-presenting capacity by direct suppression of pulmonary DC. This immunosuppressive effect of corticosteroids on DC may, however, be abrogated by exogenous GM-CSF. Corticosteroids and GM- CSF are therapeutic agents with potent direct immunomodulating effects on DC.
Source Title: Stem Cells
URI: http://scholarbank.nus.edu.sg/handle/10635/133026
ISSN: 10665099
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

5
checked on Jan 21, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.