Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1135(99)00190-X
Title: Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida
Authors: Mitchison, M.
Wei, L.
Kwang, J. 
Wilkie, I.
Adler, B.
Keywords: Immunization
Oma87
Pasteurella multocida
Issue Date: 1-Mar-2000
Citation: Mitchison, M., Wei, L., Kwang, J., Wilkie, I., Adler, B. (2000-03-01). Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida. Veterinary Microbiology 72 (1-2) : 91-96. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1135(99)00190-X
Abstract: The outer membrane protein of Oma87 from Pasteurella multocida A:1 has significant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida serotype D strain were cloned into a pGEX expression vector and transformed into E. coli JM105. Western blot analysis revealed that convalescent chicken sera reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST-F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A. Copyright (C) 2000 Elsevier Science B.V.
Source Title: Veterinary Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/132891
ISSN: 03781135
DOI: 10.1016/S0378-1135(99)00190-X
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