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|Title:||Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida|
|Citation:||Mitchison, M., Wei, L., Kwang, J., Wilkie, I., Adler, B. (2000-03-01). Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida. Veterinary Microbiology 72 (1-2) : 91-96. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1135(99)00190-X|
|Abstract:||The outer membrane protein of Oma87 from Pasteurella multocida A:1 has significant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida serotype D strain were cloned into a pGEX expression vector and transformed into E. coli JM105. Western blot analysis revealed that convalescent chicken sera reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST-F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A. Copyright (C) 2000 Elsevier Science B.V.|
|Source Title:||Veterinary Microbiology|
|Appears in Collections:||Staff Publications|
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