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|Title:||Fertilization, cleavage, and cytogenetics of 48-hour zona-intact and zona- free human unfertilized oocytes reinseminated with donor sperm|
|Authors:||Bongso, A. |
Chui Yee Fong
|Citation:||Bongso, A., Chui Yee Fong, Ng, S.C., Ratnam, S. (1992). Fertilization, cleavage, and cytogenetics of 48-hour zona-intact and zona- free human unfertilized oocytes reinseminated with donor sperm. Fertility and Sterility 57 (1) : 129-133. ScholarBank@NUS Repository.|
|Abstract:||Objective: To examine the fertilization rates of 48-hour unfertilized oocytes inseminated with fertile donor sperm and to evaluate the cleavage and cytogenetics of ensuing embryos. Design: Prospective. Setting: Assisted reproductive technology (ART) program. Patients: Four hundred ninety-seven unfertilized oocytes from 97 ART patients were categorized into four groups. A (zona-intact) and B (zona-free) were from patients with partial fertilization failure, whereas C (zona-intact) and D (zona-free) were total fertilization failures. Results: Fertilization rates in groups A and B were significantly higher than C and D (33.2% to 60.9% versus 20.0% to 48.1%; P < 0.01). Zona-free oocytes had higher fertilization rates than zona-intact oocytes (48.1% to 60.9% versus 20.0% to 32.2%). Multiple pronuclei were high in zona-free oocytes (33.1% to 41.3%). Forty-eight to 54% of embryos generated after donor insemination had chromosome anomalies (mosaicism, aneuploidy, pulverization). Conclusions: One cause of total fertilization failure appears to lie in intrinsic oocyte problems confined to the zona and oolemma. The fertilization of 48-hour unfertilized oocytes may be of some value in diagnosing fertilization failure in ART patients.|
|Source Title:||Fertility and Sterility|
|Appears in Collections:||Staff Publications|
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