Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep37235
Title: Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta
Authors: Tan, Kenneth Wei Min
Lin, Huixin
Shen, Hui
Lee, Yuan Kun
Keywords: Nitrogen depletion
Dunaliella tertiolecta
Starch
Fatty acid
Triacylglycerol
Tricarboxylic acid cycle
Microalgae
Lipid production
Issue Date: 16-Nov-2016
Publisher: Nature Publishing Group
Source: Tan, Kenneth Wei Min, Lin, Huixin, Shen, Hui, Lee, Yuan Kun (2016-11-16). Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta. Scientific Reports 6 : 1-13. ScholarBank@NUS Repository. https://doi.org/10.1038/srep37235
Series/Report no.: 6;37235
Abstract: Certain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this study, the microalga Dunaliella tertiolecta was used as a model to study the global gene expression profile regulating starch accumulation in microalgae. D. tertiolecta, when depleted of nitrogen, produced only 1% of dry cell weight (DCW) in neutral lipids, while starch was rapidly accumulated up to 46% DCW. The increased in starch content was accompanied by a coordinated overexpression of genes shunting carbon towards starch synthesis, a response not seen in the oleaginous microalgae Nannochloropsis oceanica, Chlamydomonas reinhardtii or Chlorella vulgaris. Genes in the central carbon metabolism pathways, particularly those of the tricarboxylic acid cycle, were also simultaneously upregulated, indicating a robust interchange of carbon skeletons for anabolic and catabolic processes. In contrast, fatty acid and triacylglycerol synthesis genes were downregulated or unchanged, suggesting that lipids are not a preferred form of storage in these cells. This study reveals the transcriptomic influence behind storage reserve allocation in D. tertiolecta and provides valuable insights into the possible manipulation of genes for engineering microorganisms to synthesize products of interest.
Source Title: Scientific Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/132280
ISSN: 20452322
DOI: 10.1038/srep37235
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