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|Title:||Expression of Lewisb blood group antigen in Helicobacter pylori does not interfere with bacterial adhesion property|
|Citation:||Zheng, P.-Y., Hua, J., Ng, H.-C., Yeoh, K.-G., Bow, H. (2003-01-15). Expression of Lewisb blood group antigen in Helicobacter pylori does not interfere with bacterial adhesion property. World Journal of Gastroenterology 9 (1) : 122-124. ScholarBank@NUS Repository.|
|Abstract:||Aim: The finding that some Helicobacter pylori strains express Lewis b (Leb) blood group antigen casts a doubt on the role of Leb of human gastric epithelium being a receptor for H. pylori. The aim of this study was to determine if expression of Leb in H. pylori interferes with bacterial adhesion property. Methods: Bacterial adhesion to immobilized Leb on microtitre plate was performed in 63 H. pylori strains obtained from Singapore using in vitro adherence assay. Expression of Lewis blood group antigens was determined by ELISA assay. Results: Among 63 H. pylori strains, 28 expressed Leb antigen. In vitro adhesion assay showed that 78.6 % (22/28) of Leb-positive and 74.3 % (26/35) of Leb-negative H. pylori isolates were positive for adhesion to immobilized Leb coated on microtitre plate (P=0.772). In addition, blocking of H. pylori Leb by prior incubation with anti-Leb monoclonal antibody did not alter the binding of the bacteria to solid-phase coated Leb. Conclusion: The present study suggests that expression of Leb in H. pylori does not interfere with the bacterial adhesion property. This result supports the notion that Leb present on human gastric epithelial cells is capable of being a receptor for H. pylori.|
|Source Title:||World Journal of Gastroenterology|
|Appears in Collections:||Staff Publications|
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