Please use this identifier to cite or link to this item: https://doi.org/10.1007/s002530051470
Title: Mechanism for phenol tolerance in phenol-degrading Comamonas testosteroni strain
Authors: Yap, L.F.
Lee, Y.K. 
Poh, C.L. 
Issue Date: 1999
Source: Yap, L.F., Lee, Y.K., Poh, C.L. (1999). Mechanism for phenol tolerance in phenol-degrading Comamonas testosteroni strain. Applied Microbiology and Biotechnology 51 (6) : 833-840. ScholarBank@NUS Repository. https://doi.org/10.1007/s002530051470
Abstract: Comamonas testosteroni P15 and its mutant strain E23 can tolerate and utilize phenol as the sole source of carbon and energy at up to 15 mM and 20 mM, respectively. Compared to the wild type P15, mutant E23 showed higher values of K(s) and K(i) but a lower μ(max) value, and had lower phenol hydroxylase and catechol 2,3-dioxygenase activities. Without phenol exposure, mutant E23 demonstrated a two-fold greater amount of cardiolipin than the wild type P15. Upon exposure to phenol, an increase in cardiolipin at the expense of phosphatidylethanolamine was observed in the wild type P15. However, there was no significant difference in major phospholipid contents between mutant E23 cells grown in the presence or absence of phenol. It was noted that the ratio of trans/cis fatty acids of phosphatidylethanolamine and cardiolipin in mutant E23 was 65-70% higher than that in the wild type P15. In the absence of phenol, the degree of saturation of cardiolipin in mutant E23 was 33% higher than that in wild type P15. In contrast to earlier findings, an increase in C 16:1 9trans with a simultaneous decrease in C18:1 11cis instead of C16:1 9cis was observed in specific classes of phospholipids.
Source Title: Applied Microbiology and Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/131261
ISSN: 01757598
DOI: 10.1007/s002530051470
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

25
checked on Jan 24, 2018

Page view(s)

5
checked on Jan 21, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.